OPERATED BY RS
Laboratories for Chemical and Radiochemical Synthesis
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Laboratories for Biological Studies
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These facilities include three laboratories (SQ1, SQ2, SQ3) dedicated to the synthesis of novel non-radioactive molecules to be applied in the development of molecular/nanosized tools for application in nuclear imaging (PET or SPECT) or radionuclide therapy, namely for cancer theranostics.
The research activities developed in these facilities are focused on the synthesis of organic compounds, and inorganic or organometallic complexes of d- and f- transition elements. Organic compounds include biologically active molecules and bifunctional chelators, with or without biologically active fragments, to prepare the stable d- and f- transition metal complexes.
All the synthesized compounds are characterized by multinuclear NMR spectroscopy, X-ray crystallography, mass spectrometry and HPLC.
Fume cupboards with nitrogen and vacuum lines
Rotary evaporator
Vacuum lines
Rotary Evaporators
Analytical HPLC
SQ1 - Paula Cabral Campello 
219946233
SQ2 - Célia Fernandes 219946226
SQ3 - João Galamba Correia 219946233
This Laboratory is dedicated to the solid phase synthesis, purification (semi-preparative HPLC) and characterization of biologically active peptides, taking advantage of a CEM-Liberty microwave automated peptide synthesizer.
CEM Liberty Automated Microwave Peptide Synthesizer and HPLC: UV/Vis detector
CEM-Liberty microwave automated peptide synthesizer
Semi-preparative HPLC
João Galamba Correia 219946233
In this laboratory biodistribution studies of radioactive probes are carried out in healthy or tumor bearing mice/rats. Pharmacokinetics and metabolic studies are also performed. This facility also includes an establishment for housing and care of small rodents with control of temperature, humidity and light, approved by the National Authorities.
Left - Individual ventilated cage system (IVC); Right - Intravenous administration of a radiocompound for biodistribution studies
Left - Dose calibrator; Right - Multi-channel -counter
Animal housing facilities
Individual ventilated cage system (IVC)
Fume hoods for animal injection and biodistribution
Dedicated dose calibrator
Multi-channel -counter
Lurdes Gano 219946195
These laboratories are under negative pressure and are equipped with hot cells, glove boxes and fume hoodsthat allow for the safe handling of radioactive compounds.
These facilities are also provided with High Performance Liquid Chromatography Systems (HPLC) coupled to UV-vis and Gamma Detectors, a Thin Layer Chromatography Scanner and an Electrophoresis Apparatus dedicated to the characterization and quality control of the radioactive compounds,
This infrastructure is also equipped with radiation monitors, dose calibrators and gamma counters for safety control and measurement of radioactivity. These laboratories are regularly used for training on radiopharmacy (students from ESTeSL and resident physicians in Nuclear Medicine)
Laboratories for the Synthesis of Radiocompounds Hand and foot radiation monitor
Hot cells dedicated to radiosynthesis
Glove boxes dedicated to the radiosynthesis of radiohalogenated compounds and Analytical HPLC coupled to and UV detector
Training on radiopharmacy
Analytical HPLC coupled to and UV detectors (2)
Thin Layer Chromatography Scanner
Electrophoresis Apparatus
Dose calibrator
Multi-channel -counter
António Paulo 219946197
This facility includes a laboratory for Cell Culture and a laboratory for In Vitro Studies of radioactive compounds
The Cell Culture laboratory is equipped with CO2 incubators, biohazard cabinets, a low temperature freezer (-80ºC) and a refrigerated centrifuge with conventional rotors, and inverted and conventional optical microscopes. The laboratory for In Vitro Studies of radioactive compounds is equipped with a fume hood, instruments for radiation measurement and a microplate UV-vis spectrophotometer.
In the Cell Culture laboratory a small collection of primary and cancer cell lines of animal or human origin is used to evaluate non-radioactive and radioactive compounds. The cell studies include: cytotoxicity and radiotoxicity assays, uptake, internalization, externalization and subcellular distribution studies as well as immunoreactivity, receptor affinity and mechanistic studies.
CO2 Incubator and Biohazard Cabinets
Left - Refrigerated centrifuge with conventional rotor; Right - Optical microscope
Microplate UV-vis spectrophotometer
Fume cupboard for radioactive binding assays
CO2 incubators
Biohazard cabinets
Low temperature freezer (-80ºC)
Refrigerated centrifuge with conventional rotors
Inverted and conventional optical microscopes
Filipa Mendes 219946196